BD模拟脂质纳米孔的脂质扰动。这部电影显示了静止微观配置的集合,说明了L = 24 nm系统的200 µs仿真轨迹。同一仿真系统的剪切和顶视图分别显示在顶部和底部面板中。两个面板中的红白色蓝色背景代表模拟中应用的位置依赖性电位,以说明环形孔的效果。顶部面板上的颜色图显示了系统的Y - z横截面,x = 0 nm;底部的一个是z = 2 nm处的x -y横截面。电位的颜色尺度显示在右侧面板上。底部面板中的绿色虚线表示孔的位置。黄色和黑色球形描绘了荧光和非荧光脂质。最初(在t = 0)中,降低了双层下部小叶(z <0 nm)中所有脂质的荧光。 During the simulation, lipids from the upper leaflet (Z > 0 nm) diffuse to the lower leaflet where they are reduced in their fluorescence (yellow beads turn black). The “Ratio” variable shows the ratio of the beads remaining in the upper leaflet that have never ventured to the lower leaflet from the beginning of the simulation to the total number of beads in the upper leaflet.